Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection. Glucose metabolism plays a fundamental role in supporting the growth, proliferation and effector functions of T cells. We investigated the impact of HIV infection on key processes that regulate glucose uptake and metabolism in primary CD4+ and CD8+ T cells. Thirty-eight HIV-infected treatment-naïve, 35 HIV+/combination antiretroviral therapy, 7 HIV+ long-term nonprogressors and 25 HIV-control individuals were studied. Basal markers of glycolysis [e.g. glucose transporter-1 (Glut1) expression, glucose uptake, intracellular glucose-6-phosphate, and L-lactate] were measured in T cells. The cellular markers of immune activation, CD38 and HLA-DR, were measured by flow cytometry.
GlobalResearch at UCSF presents the broad scope of health research that is being conducted by UCSF researchers worldwide